Effect of Broad-Spectrum Hemp Extract on Neurobehavioral Activity on the Immobilization Stress-Induced Model in Sprague Dawley Rats
A broad-spectrum, nondetectable THC hemp extract with other minor cannabinoids (broad-spectrum hemp extract) was administered at 28 mg/kg·bw to evaluate its anxiolytic properties. It was performed using various behavioral models and biomarkers for oxidative stress. In addition, a 300 mg/kg·bw of Ashwagandha root extract was also incorporated to compare its effects on relieving stress and anxiety. The study was conducted using 24 adult male SD rats that were randomly divided into four groups: Group 1 (G1): induction control and no treatment were given; Group 2 (G2): placebo medium chain triglycerides (MCT oil); Group 3 (G3): broad-spectrum hemp extract (28 mg·kg−1·bw) for 4 weeks; and Group 4 (G4): Ashwagandha (300 mg·kg−1·bw) for 4 weeks. Dose formulations were administered to the specific treatment groups for 28 consecutive days. This study showed that CBD was comparable to Ashwagandha in modifying the behaviour of the rodents as exhibited in the test results from the open-field and swim despair tests and improving oxidative stress parameter scores.
