The study aimed to evaluate the effect of VagiBIOM, a multi-Lactobacillus vaginal suppository, on perimenopausal women with bacterial vaginosis (BV) in restoring vaginal pH and overall vaginal health. Sixty-six women with BV symptoms were randomized to receive VagiBIOM or placebo suppositories. After 4 weeks of intervention, VagiBIOM significantly decreased vaginal pH, VAS itching score, and total Nugent score compared to baseline. The overall vaginal health index (VHI) significantly improved following the 28-day intervention with VagiBIOM.